Annex Publishers

Penicillin and streptomycin are the most commonly used to prevent bacterial infection or contamination in cell culture. However, the role of Penicillin-streptomycin in inducing mouse embryonic stem cells (mESCs) to differentiate into cardiomyocytes is unclear. To illustrate the mechanism of penicillin-streptomycin on inducing mESCs differentiation into cardiomyocytes, we firstly investigated mESCs differentiation into cardiomyocytes by the traditional method. e timespace expressions of cardiac-specific genes were detected by real-time PCR and western blotting at 8 different time-points over a period of 36 days.

Objective: The aim of this project consists of the evaluation of the cell viability of adipose tissue (AT) harvested using the Superficial Enhanced Fluid Fat Injection (SEFFI) procedure standardized by the Authors (AG, FPB). The harvesting procedure was performed using two different cannulas having 0.8 mm and 1 mm side-port holes, respectively. Cells phe- notype and ability to adhere to the plastic surface have been analysed. 'Ihe results have been compared to those recorded in adipose tissue harvested using a liposuction system and processed with enzymatic digestion (collagenase).

Abstract Purpose: In this article, we review main clinical trials reported in the past decade. Methods: Stem cells are relatively undifferentiated, with unlimited proliferative ability; self-renewal capability and also they can differentiate into specialized cells. Somatic stem cells in adult organisms are responsible for regenerating and self-renewing tissue, Many different stem cell types reside in the eye. Results: One of the most important stem cells is limbal stem cell that retains in an undifferentiated state and exists in an optimal microenvironment or "niche". The importance of limbal stem cells in maintaining the corneal epithelium throughout life has long been recognized. Furthermore, stem cells in the retina have been for treatment of the retinal degeneration, which generally results in constant visual disturbance or even blindness. Conclusion: This review will briefly focus on the principal stem cells in the eye especially limbal stem cells.

Introduction: Buccal mucosal urethroplasty has revolutionized the surgical management of urethral strictures but the success of the procedure has been variable in different series. We have attempted to understand the interplay between buccal mucosal epithelial cells (BME) and the fibroblasts at the molecular level using connective tissue growth factor (CTGF) as a marker so that translation of this information would help in better urethroplasty outcomes.