Degradation of sesaminol triglucoside in sesame milk fermentation by β-glucosidase producing Lactobacillus plantarum Dad 13

Sesaminoltriglucosideis a bioactive compound in sesame milk which has antioxidant activity. Sesaminoltriglucoside exhibits higher antioxidant activity when it is hydrolyzed by β-glukosidase. The aims of this research are to study the increase ofantioxidant activity and the decrease of sesaminoltriglucoside concentration during sesame milk fermentation by L. plantarumDad 13.Sesame milk was inoculated with L. plantarum Dad 13 and incubated at 37oC for 18 h. The viable cell, β-glucosidase activity, sesaminol triglucoside concentration and antioxidant activity were monitored .during fermentation. The crude extract of sesaminolglucosidelignan from defatted sesame seed was hydrolyzed using β-glucosidase. The antioxidant activity and the decrease of sesaminol triglucoside were analyzed. The results showed that L. plantarum Dad 13 grew well in sesame milk fermentation and produced β-glucosidase during fermentation.The antioxidant activity of sesame milk fermentation increased 2.34 times and sesaminol triglucoside concentartion decreased 56,4%. Hydrolysis of β-glucosidase on sesaminolglucosidelignan crude extract resulted in decrease of sesaminoltriglucoside concentration and increase its antioxidant activity. It can be concluded that the increase of antioxidant activity was due to the degradation of sesaminoltriglucosideby β-glucosidasethat produced by L. plantarum Dad 13.

Dr. Ulyatu Fitrotin is currently working at The Assessment Institutes for Agricultural Technology of West Nusa Tenggara, Indonesia. He published many articles in reputed journals and attended many international conferences.