 |
Figure 1: GSH Standard Curve (OD) was taken at 412 nm |
|
Figure 1: GSH Standard Curve (OD) was taken at 412 nm |
Standardized Botanical Extract |
Concentrations of Standardized Botanical Extracts and Reference Standard (Ascorbic acid) |
% DPPH Radical Inhibition |
1%Huperzia serrata Extract (CogniUp) |
0.125 μg/mL |
28.2 + 0.5 |
Convolvulus pluricaulis - (SP) |
12.0 mg/mL |
24.5 + 2.1 |
Celastrus paniculatus- (JY) |
4.0 mg/mL |
16.2 + 0.5 |
CogniUp + SP + JY |
0.125 mg/mL + 12.0 mg/mL + 4.0 mg/mL |
31.5 + 0.3** |
Ascorbic Acid |
0 - 100 μg/mL (Reference Standard) |
- |
The % DPPH Radical Inhibition, showing antioxidant efficacy of different standardized botanical extracts of CogniUp, SP, JY and a combination of CogniUp + SP + JY was estimated using DPPH radical inhibition assay. **p < 0.001).
Table 1: % DPPH Radical Inhibition by Standardized Huperzia serrata (CogniUp, 1% Huperzine A), Convolvulus pluricaulis (SP) and Celastrus paniculatus (JY) Extracts on 1,1-diphenyl-2-picrylhydrazyl (DPPH) Radical
Samples (Concentration) |
Reduced (GSH) Content (𝛍M) |
Control Buffer |
327 + 18.6 |
H202 (600 μM) |
88.5 + 7.5* |
1%Huperzia serrata Extract (CogniUp) (0.125 μg/mL) |
347 + 18.7** |
Convolvulus pluricaulis - (SP) (12 mg/mL) |
328.9 + 20.5** |
Celastrus paniculatus- (JY)(4 mg/mL) |
339.1 + 31.2** |
CogniUp (0.125 μg/mL) + SP (12 mg/mL) + JY (4 mg/mL) |
337.5 + 24.8** |
CogniUp (0.125 μg/mL) + H202 (600 μM) |
308.3 + 26.7** |
SP + H202 (600 μM) |
250.5 + 21.2** |
JY + H202 (600 μM) |
284.4 + 24.3** |
CogniUp (0.125 μg/mL) + SP (12 mg/mL) + JY (4 mg/mL) + H202 (600 μM) |
328.5 + 30.7** |
GSH depletion of different standardized botanical extracts of CogniUp, SP, JY and a combination of CogniUp + SP + JY was estimated against H2O2-induced GSH depletion assay. *p < 0.05; **p < 0.001
Table 2: Comparative Protective Effects of SP, JY and CP Against GSH Depletion
Samples (Concentration) |
AChE Inhibition (%) |
1%Huperzia serrata Extract (CogniUp) (0.125 μg/mL) |
30.18% |
Convolvulus pluricaulis - (SP) (12 mg/mL) |
27.63% |
Celastrus paniculatus- (JY)(4 mg/mL) |
25.86% |
CogniUp (0.125 μg/mL) + SP (12 mg/mL) + JY (4 mg/mL) |
37.3% |
CogniUp (0.25 μg/mL) + SP (12 mg/mL) + JY (4 mg/mL) |
33.84% |
Acetylcholinesterase (AChE) inhibition of different standardized botanical extracts of CogniUp, SP, JY and a combination of CogniUp + SP + JY was estimated
Table 3: Comparative Protective Effects of SP, JY and CP Against AChE Inhibition