Rat Blood Cell Sample

Reduced Glutathione (GSH)*

Glutathione Reductase (GR)*b

Glutathione Peroxidase (GPx)*b

mg/100mL RBC

mg/g Hb

IU/g of Hb

IU/g of Hb

Control

24.42 ±1.18

1.58 ±0.121

7.34 ±0.34

25.23 ±1.02

Indomethacin

20.44 ±0.90a

1.426 ±0.007a

7.38 ±0.39

25.90 ±0.91

Indomethacin + Vitamin E

23.54 ±0.30a

1.487 ±0.045

7.85 ±0.40

24.95 ±1.21

* Each value is the mean of at least 3-4 experiments ±SD
a P< 0.001; highly significant when compared with control
b Enzyme activity is expressed in terms of IU (µmole of NADPH oxidized/minute) per g of hemoglobin at 37^oC
Table 1: Reduced glutathione (GSH), Glutathione reductase (GR) and Glutathione peroxidase (GPx) levels in Red
Blood Sample of Control, Indomethacin-treated and Indomethacin with Vitamin E-Treated Rats

Rat Blood Cell Sample

Acetylcholinesterase (AChE) Activity*b

(Na+,K+)-ATPase Activity*c

IU/g of Hb

µmole Pi/mg Protein

Control

16.55 ±1.27

0.162 ±0.010

Indomethacin

29.42 ±0.96a

0.168 ±0.008

Indomethacin + Vitamin E

27.06 ±1.71

0.166 ±0.006

* Each value is the mean of at least 3-4 experiments ±1SD
a P< 0.001; highly significant when compared with control
b Enzyme activity is expressed in terms of IU (µmole of acetyl thiocholine iodide hydrolyzed/minute) per g of hemoglobin at 37^oC
c Enzyme activity is expressed in terms of µmole Pi liberated per hour per mg of protein.
Table 2: Red cell membrane Acetylcholinesterase (AChE) and (Na+,K+)-ATPase Activities of Control, Indomethacin treated and Indomethacin with Vitamin E Treated Rats

% Hemolysis

% NaCl

Control (No Drug Treatment)

Indomethacin

Indomethacin + Vitamin E

0.1

98.91 ±0.78

98.29 ±1.38

98.74 ±0.70

0.2

97.23 ±2.23

94.16 ±1.59

94.53 ±0.52

0.3

100 ±0.00

100 ±0.00

100 ±0.00

0.4

97.64 ±2.05

97.83 ±0.96

96.89 ±0.80

0.5

96.27 ±1.16

96.18 ±2.04

94.98 ±1.77

0.6

92.2 ±1.64

92.69±2.84

92.84 ±2.68

0.65

92.27 ±3.54

85.86 ±3.17

86.96 ±3.59

0.7

76.39 ±3.94

59.49 ±2.59

66.07 ±15.19

0.75

43.80 ±7.87

24.05 ±3.88

36.29 ±9.00

0.8

14.22 ±4.98

6.78±0.77

8.65 ±1.36

0.85

4.34 ±0.97

1.43 ±0.96

2.79 ±0.47

0.9

0.00 ±0.00

0.00 ±0.00

0.0 0.00

Severe acute malnutrition (<11.5cm)

78

68.5

Erythrocyte osmotic fragility was assessed as a % Hemolysis of control, Indomethacin-treated and Indomethacin + vitamin E-treated
rats. % Hemolysis was assessed at various concentrations of NaCl. See Materials and Methods section for details.
Table 3: Erythrocyte Osmotic Fragility (% Hemolysis) of Control, Indomethacin treated and Indomethacin with Vitamin E Treated Rats