Figure 1: Erythrocyte Osmotic Fragility Profile of Control, Acetylsalicylic acid (ASA) treated and Acetylsalicylic acid (ASA) with Vitamin E Treated RatsT

Rat Blood sample	Reduced Glutathione (GSH)*		Glutathione Reductase *(GR)^b**	*Glutathione Peroxidase(GPx)^b**
	mg/100ml RBC	mg/g Hb	IU/g Hb	IU/g Hb
Control	24.76 ±0.50	1.618 ±0.076	7.42 ±0.37	25.18 ±1.10
Acetylsalicylic acid (ASA)	10.64 ±0.07^a	0.841 ±0.015^a	7.48 ±0.15	26.13 ±1.32
Acetylsalicylic acid (ASA)+Vitamin E	23.65 ±0.32	1.544 ±0.076	7.92 ±0.32	25.61 ±1.16
- Each value is the mean of at least 3-4 experiments ±SD a- P< 0.001; highly significant when compared with control b- Enzyme activity is expressed in terms of IU (µmole of NADPH oxidized/minute) per g of hemoglobin at 37 ºC Table 1:* Reduced Glutathione (GSH), Glutathione Reductase (GR) and Glutathione Peroxidase (GPx) Levels of Control, Acetylsalicylic acid (ASA) treated and Acetylsalicylic acid (ASA)+Vitamin E Treated Rats

Rat Blood Sample	*Acetylcholinesterase (AChE) Activity^b (IU/g Hb)**	Na+, K+-ATPase *Activity^c (µmole Pi/mg Protein)**
Control	18.99 ±1.29	0.163 ±0.023
Acetylsalicylic acid (ASA)	11.35 ±1.04^a	0.188 ±0.014
Acetylsalicylic acid (ASA)+Vitamin E	18.98 ±2.15	0.182 ±0.006
- Each value is the mean of at least 3-4 experiments ±SD a- P< 0.001; highly significant when compared with control b- Enzyme activity is expressed in terms of IU (µmole of acetyl thiocholine iodide hydrolyzed/minute) per g of hemoglobin at 37 ºC c- Enzyme activity is expressed in terms of µmole Pi liberated per hour per mg of protein. Table 2:* Red cell membrane Acetylcholinesterase (AChE) and Na+, K+-ATPase Activities of Control, Acetylsalicylic acid (ASA) treated and Acetylsalicylic acid (ASA)+Vitamin E Treated Rats

% Hemolysis
% NaCl	Control	Acetylsalicylic acid (ASA)	Acetylsalicylic acid (ASA)+Vitamin E
0.1	91.95 ±1.63	97.98 ±0.62	93.61 ±2.17
0.2	90.72 ±2.32	96.71 ±1.54	93.90 ±3.94
0.3	100 ±0.00	100 ±0.00	100 ±0.00
0.4	94.77 ±0.33	99.09 ±0.15	96.51 ±0.95
0.5	90.85 ±2.75	95.87 ±0.67	94.75 ±1.91
0.6	85.60 ±0.61	93.15 ±2.65	88.61 ±0.83
0.65	81.30 ±2.04	86.47 ±5.06	73.58 ±12.09
0.7	60.35 ±0.54	66.74 ±5.61	35.97 ±17.07
0.75	23.99 ±0.72	29.58 ±8.98	12.66 ±7.91
0.8	8.29 ±3.29	9.58 ±1.16	4.14 ±2.56
0.85	4.27 ±1.46	4.60 ±1.35	1.47 ±0.83
0.9	0.00 ±0.00	0.00 ±0.00	0.00 ±0.00
Erythrocyte osmotic fragility was assessed as a % Hemolysis of control, ASA-treated and ASA+vitamin E-treated rats. %Hemolysis was assessed at various concentrations of NaCl. See Materials and Methods section for details. Table 3: Erythrocyte Osmotic Fragility (%Hemolysis) of Control, Acetylsalicylic acid (ASA) treated and Acetylsalicylic acid (ASA)+Vitamin E Treated Rats